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A mad scientist kidnaps and mutilates a trio of tourists in order to reassemble them into a human centipede, created by stitching their mouths to each others' rectums.

Americans Lindsay and Jenny are on vacation traveling through Europe. In Germany, they meet a waiter who invites them to a party in a nightclub called Bunker. They rent a car, but they get lost on a lonely road in the woods. When they have a flat tire, they do not replace the tire and prefer to seek for help instead. They reach a house, where they are received by a psychopathic mad scientist named Joseph Heiter, who drugs them. When Lindsay and Jenny awake, they find that they are tied to a bed in a laboratory in the basement of the house. Sooner, Dr. Heiter brings a Japanese tourist named Katsuro and introduces himself as a former surgeon specialized in separating Siamese twins. Further, he details his insane project of creating a human centipede by joining three human beings through their mouths and anuses.


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